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cuffdiff error: Segmentation fault: 11

I got an error from cuffdiff

 $ ~/programs/cufflinks-2.2.1.OSX_x86_64/cuffdiff -o diff_out -b ~/GRCm38_86/mouse.fa -L 67,89 -u merged_asm/merged.gtf ./106_thout/accepted_hits.bam,./107_thout/accepted_hits.bam ./108_thout/accepted_hits.bam,./109_thout/accepted_hits.bam
    You are using Cufflinks v2.2.1, which is the most recent release.
    [20:40:48] Loading reference annotation and sequence.
    [20:41:14] Inspecting maps and determining fragment length distributions.
    Segmentation fault: 11

Any suggestions ?

thanks so much~~

rna-seq
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updated by Biostar Biostar • written by jolin0701-dy jolin0701-dy

Segmentation faults are generally related to out of memory issues. Can you try the above command without -b and -u options, just to check if these parameters are causing too much memory usage.

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written by Persistent LABS Persistent LABS

you are right. It is due to low memory...Thanks~~

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written by jolin0701-dy jolin0701-dy

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