I do some circular RNA researches recently. I have some questions about the boundary of Circular RNA. Since Circular RNA caused by some exons linking back to their previous one, and the RNA detection tool will only report the left position and the right position of the latent circular RNA, is it correct for me to consider the boundary of circular RNA should come from the the boundary of exons?
For example if some of tools report the detected circular rna is (1000, 2000), then i could speculate that both 1000 and 2000 should come from the boundary of some exons.
If my assumption above is incorrect, how about another idea: the majority part of the boundary of circular RNA comes from the boundary of exons. If it is, may i know how many percentage satisfy the second assumption above?
Thanks. I am really appreciate for your helps.