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TPM for miRNAs

Hi All,

How one can calculate TPM (Transcripts per million) for miRNA tags/reads counts?

eg: 

miRNAids S_1.readcou S_2.readcou
mmu-let-7a-1-3p    421    230
mmu-let-7a-5p    68708    26836
mmu-let-7b-3p    26    7

Thanks,

Manoharan

next-gen
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updated by Biostar Biostar • written by manoharankumar01 • manoharankumar01

Hi,

Thanks for sharing the link, but that is for mRNA seq. Is it same for miRNAs as well?

Here instead of gene length should I consider miRNAs length?

Thanks,

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written by manoharankumar01 • manoharankumar01

It's the same regardless of whether it's miRNA or mRNA or total RNA or rRNA or anything else. For genes the length is that of the gene, for miRNAs that of the miRNA, for rRNAs that of the rRNA, etc.

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written by Devon Ryan Devon Ryan

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