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miRNA DE analysis with mirdeep2

hi,

I want to provide read counts file for edger analysis. then by this syntax from mirdeep2

quantifier.pl -p precursors.fa -m mature.fa -r reads.fa -s star.fa -y now -t cel

but in my organism " Aspergillus fumigatus" how I can find precursors.fa and mature.fa files????

in mirbase there is not my desire organism :( :( :(

mirnas mirdeep2 de analysis rna-seq quantifier.pl
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updated by harold.smith.tarheel harold.smith.tarheel • written by zizigolu zizigolu

1 answer

Obviously, you have to create the precursor and mature files yourself, perhaps based on synteny/sequence similarity plus transcript data.

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written by harold.smith.tarheel harold.smith.tarheel

thank you, you mean I should blast what on what please?

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written by zizigolu zizigolu

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