how to cluster sequences with uclust using multiple parameters
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7.6 years ago
User 6777 ▴ 20

Anyone has any idea on multiple accept options in uclust by combining them with AND, as detailed in their manual here: usearch manual

I want to use a usearch command like:

usearch -cluster_fast seq2.fasta -centroids seq.fasta -uc clusters.uc -id 0.8 AND ‑evalue 1e-10 AND ‑query_cov 0.8 AND ‑target_cov 0.8

Also if u do know this, plz suggest how can I use "-msaout" option in the above command.

Any suggestion is appreciated. Thanks..

next-gen clustering usearch uclust • 2.2k views
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