deseq and its negative binomial distribution
whats the role of negative binomial distribution in rna seq and how is deseq and edgeR differ from each other. please explain the statistics behind this distribution...its too complicated
rna-seq
• 2,703 views
•
link
written
by
dezhil.ezhil •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
RNA-seq counts distribution
written by ecj •Hi there, I am quite new in the bioinformatics world and I would appreciate a bit of help, please. I would like to have an …
-
Wilcoxon test, t-test and negative binomial test for DEG of scRNA-Seq data
written by CYI tried Wilcoxon test, t-test and negative binomial test across different amount of cells (biological replicates) (3, 6, 10, 50, 100, 500) derived from scRNA-Seq …
-
Why NB distribution is preferred in RNA-Seq while normal distribution is preferred in microarray?
written by CYMost of DE tools (such as DE-Seq) applied in RNA-Seq assume that gene expression follows Negative Binomial distribution (because of both technical and biological variation). …
-
Why can't use t-test for differential expression (negative binomial distribution assumed)
written by CYAccording to central limit theorem, t-test can be used for non-normally distributed sample. Beside, RNA-Seq fits better to negative binomial distribution which doesn't significantly differ …
-
differential gene expression analysis using log2(counts+1)
written by elbHi guys, I know that DESeq as well as EdgeR perform differential gene expression analysis from RNA seq experiments using counts. I have normalized my …
-
What is the actual cause of excessive zeroes in single cell RNA-seq data? Is it PCR?
written by Martin ModrákFirst, sorry if I am missing something basic - I am a programmer recently turned bioinformatician so I still don't know a lot of stuff. …
-
multivariate analysis of RNA-seq
written by moxuWhen you have only one variable with two categories (e.g. disease vs control) to compare for RNA-seq, you assume the expression level follows negative binomial …
-
Negative Binomial and Poisson distribution of RNA-Seq
written by ashwiniDear All, I am a Biologist trying to understand the statistics of RNA-Seq data. Given that RNA-Seq follows NB distribution with Biological replicates, as NBD …
-
Why Does Rna-Seq Read Count Fit Poisson Distribution?
written by sckinta<p>Although R packages like "edgeR" "DEseq" have been frequently used for RNA-seq expression study, I am still confused that why RNA-seq read count fits Poisson …
-
Statistical Distributions In Rna-Seq Data Analysis
written by Ngsnewbie •<p>I came to know that RNA-seq data follows bionomial/ negative bionomial distribution. Well i am not a statistician, but i studied about basics of statistics, …
Is this a quiz? What can I win?
Differences between DESeq and edgeR
Stephen Turner @genetics_blog http://www.gettinggeneticsdone.com/2012/09/deseq-vs-edger-comparison.html
for more background --> http://www.nature.com/nprot/journal/v8/n9/full/nprot.2013.099.html
I'm going to close this until OP can confirm that we're not doing his/her homework.