This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Running "make" fails on RHEL6 but not Ubuntu (bits/c++config.h:No such file or directory)

I'm running make to install Bowtie on RHEL 6 and I get this error:

g++ -O3 -m64 -DCOMPILER_OPTIONS="\"-O3 -m64  -Wl,--hash-style=both -DPOPCNT_CAPABILITY  \""  -Wl,--hash-style=both -DPOPCNT_CAPABILITY    \
                -fno-strict-aliasing -DBOWTIE_VERSION="\"`cat VERSION`\"" -DBUILD_HOST="\"`hostname`\"" -DBUILD_TIME="\"`date`\"" -DCOMPILER_VERSION="\"`g++ -v 2>&1 | tail -1`\"" -D_LARGEFILE_SOURCE -D_FILE_OFFSET_BITS=64 -D_GNU_SOURCE  -DPREFETCH_LOCALITY=2 -DBOWTIE_MM -DBOWTIE_SHARED_MEM -DNDEBUG -Wall \
                -I SeqAn-1.1 -I third_party -I third_party \
                -o bowtie-build-s ebwt_build.cpp \
                ccnt_lut.cpp ref_read.cpp alphabet.cpp shmem.cpp edit.cpp ebwt.cpp tinythread.cpp  bowtie_build_main.cpp \
                -lpthread
In file included from ebwt_build.cpp:1:
/usr/lib/gcc/x86_64-redhat-linux/4.4.7/../../../../include/c++/4.4.7/iostream:39:28: error: **bits/c++config.h: No such file or directory**
In file included from /usr/lib/gcc/x86_64-redhat-linux/4.4.7/../../../../include/c++/4.4.7/ios:42,
                 from /usr/lib/gcc/x86_64-redhat-linux/4.4.7/../../../../include/c++/4.4.7/ostream:40,

=============

Running RHEL6 and I installed bowtie with a 

git clone https://github.com/BenLangmead/bowtie

I open up the tutorial and execute the following command:

$ ./bowtie e_coli reads/e_coli_1000.fq

And get this error

Traceback (most recent call last):
  File "./bowtie", line 70, in <module>
    main()
  File "./bowtie", line 66, in main
    os.execv(bin_spec, arguments)
OSError: [Errno 2] No such file or directory

EDIT: make errored out on RHEL but not Ubuntu.

bowtie
• 5,551 views • link
updated by tomkinsc • tomkinsc • written by jnowacki jnowacki

2 answers

So this works on my Ubuntu machine but not RHEL6

Seems like the problem is the make command errors out on RHEL.

yum install gcc

does not remedy the problem.

• 1 views • link
written by jnowacki jnowacki

If this is a cross-compilation issue (i.e. compiling for 64 bits on a 32-bit platform), it may help to install the multilib libraries mentioned in the link posted by microfuge. More likely is that it's an issue with the older gcc, in which this subcomment of that link may help. The gist of it is this:

cd /usr/include/c++/4.4.7/
mkdir x86_64-redhat-linux
cd x86_64-redhat-linux
ln -s ../i686-redhat-linux 32

If you an get away with a pre-compiled version, I'd suggest installing from bioconda.

After installing conda, it's a simple command to install bowtie:

conda install bowtie
• 1 views • link
written by tomkinsc • tomkinsc

Log in to answer this question.

More posts like this