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Paired end FASTA to single BAM file

Hi,

I'm interested in taking two fastq files X_1.fq and X_2.fq creating a single bam file X.bam, unmapped to anything. Is there a way to do this with just bowtie2 and samtools? Thank you for the help!

samtools

Just curiously, what are reasons one would do this?

1 answer

Would Picard be acceptable? You can do it easily with FastqToSam: https://broadinstitute.github.io/picard/command-line-overview.html#FastqToSam

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