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Finding differentially expressed genes between samples from different studies

I want to find differentially expressed genes between samples from different datasets in GEO database (not between samples from the same dataset) by Babelomics’ tools or other free and easy to use tools?

differntially expressed genes
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written by majzoub.ruba • majzoub.ruba

So you want to compare treatment A in experiment 1 with treatment B in experiment 2? That might give you invalid results, since you cannot determine whether the differential expression is due to a) the treatment or b) a batch effect between the two experiments.

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written by WouterDeCoster WouterDeCoster

I mean: For example, I want to compare the expression profile of an embryonic stem cell from a study with the expression profile of an induced pluripotent stem cell from another study to find the differentially expressed genes between them.

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written by majzoub.ruba • majzoub.ruba

Then WouterDeCoster is correct - experiment and cell type are confounded and you cannot reliably detect differences between them. This would only work if you had both cell types for both studies.

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written by keith.hughitt keith.hughitt

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