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how to find significant genes using biological replicate

Here is my real data

I have 3 control samples and 3 treated samples. the data is LFQ expression means it is continuous values. One should normalise it for example take log from the data and i should mention that two of them are biological replicate. I am looking to find a way to determine significance changes between these samples using R? Probably using t test or kolmogorov-smirnov or any other test which is valid

r

Can't you just do the experiment yourself?

You can just but the header of file

2 answers

Have you checked previous discussion on the topic?

@igor Yes I did, those answer are mainly based on packages and also the first one is for SILAC. this one is bit different. I know limma is a good package to work with and I have no problem with it. I am more into other ideas rather than packages

It may help if you describe what this data is. Many are not going to be familiar with LQF (label free quantification) and what the numbers in this table represent. Perhaps you will get better ideas for analysis then.

You could also keep just a few example rows in your github source (rather than the full table) to make this post more readable for others.

I'm not familiar with LFQ expression data but it looks like counts so you can use the R package DESeq2.

@Carlo Yague if you don't know then you must not just post an answer but a comment. no DESeq2 is for count values and it is not. moreover i am not looking for packages , otherwise there are some out there , I am more looking for other solutions

Are these numbers from Maxquant (LFQ - label free quantification/)?

@genomax2 yes, they are

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