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Analyse RNA-seq differential expression data

Sorry in advance, I'm sure this question has already been posted before, but I haven't been able to find a satisfying answer.

I have to analyse RNA-seq differential expression data, and I've never done that before. So I have a big list of genes with different FDR and log fold change.

What is your method to extract relevant information from this? I've read that just putting arbitrary threshold is not a good solution, but I don't really know how to filter those results...

Any advice is more than welcome!

rna-seq differential expression

1 answer

FDR adjusted p-value < 0.05 is pretty standard. I am not a big fan of Fold change cutoffs, but a 1.5 or 2 fold change cutoff is often found in literature.

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