Analyse RNA-seq differential expression data
Sorry in advance, I'm sure this question has already been posted before, but I haven't been able to find a satisfying answer.
I have to analyse RNA-seq differential expression data, and I've never done that before. So I have a big list of genes with different FDR and log fold change.
What is your method to extract relevant information from this? I've read that just putting arbitrary threshold is not a good solution, but I don't really know how to filter those results...
Any advice is more than welcome!
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FDR adjusted p-value < 0.05 is pretty standard. I am not a big fan of Fold change cutoffs, but a 1.5 or 2 fold change cutoff is often found in literature.
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Hi, You can check this, too:
https://groups.google.com/forum/#!searchin/trinityrnaseq-users/fdr|sort:relevance/trinityrnaseq-users/T9VBfIAMbjU/VPMoYWfpEQAJ
bonne chance!