GTEx RNA-Seq GTEx_Analysis_v6_RNA-seq_RNA-SeQCv1.1.8_gene_rpkm.gct.gz
hi,
I have downloaded GTEx_Analysis_v6_RNA-seq_RNA-SeQCv1.1.8_gene_rpkm.gct.gz from the GTEx portal. May i know if i wanted to calculate the fold-change among the samples, should i normalize the RPKM file again (probably using TMM edgeR or so)? Or can i just straight away use this GTEx_Analysis_v6_RNA-seq_RNA-SeQCv1.1.8_gene_rpkm.gct.gz's value to calculate the fold change? does anyone has experience in doing this?thanks very much :)
J
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