This is a test version of Biostars. For the public version, visit https://www.biostars.org.
GTEx RNA-Seq GTEx_Analysis_v6_RNA-seq_RNA-SeQCv1.1.8_gene_rpkm.gct.gz

hi,

I have downloaded GTEx_Analysis_v6_RNA-seq_RNA-SeQCv1.1.8_gene_rpkm.gct.gz from the GTEx portal. May i know if i wanted to calculate the fold-change among the samples, should i normalize the RPKM file again (probably using TMM edgeR or so)? Or can i just straight away use this GTEx_Analysis_v6_RNA-seq_RNA-SeQCv1.1.8_gene_rpkm.gct.gz's value to calculate the fold change? does anyone has experience in doing this?thanks very much :)

J

rna-seq

Posting name of a file as the title of a post conveys no useful information. Please consider changing the title to something more meaningful.

0 answers

No answers yet.

Log in to answer this question.