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How to calculate dN/dS values?

Hello,

I have made a codon alignment using pal2nal and generated a .codon file. Now I want to calculate the dN/dS values using paml. How can I generate tree file (.tree) and (.cnt) to be used by codeml in paml? If I am doing something wrong please guide me for the correct method to calculate dN/dS values.

Thanks in advance!

genome dn/ds paml pal2nal

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