@Prasad - It would be better if you change it answer from comment and format the code, so that OP can accept the answer and the question will not remain as unanswered, and also helpful for future googlers.
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use the sortBed
can you tell me how should i sort using sortbed i mean commands
i used bedtool/sort -chrThenSizeA -i my.bed > new.bed
it not working
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