Is normalization mandatory for read counts in RNA-seq experiments samples?
I don't have any replicates of my RNA-Seq sample, is it mandatory to normalize the read counts? because when I used NOISeq QC report, it reported that without any normalized method diagnostic method is passed.
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Devon Ryan
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Bioblazer
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Bioblazer
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Some form of normalization is more or less always needed. Having said that, with no replicates the results of any method will be at best of marginal utility.
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