Does SPades contigs.fasta contain unassembled reads?
Hopefully this is a simple question! I'm performing de novo assembly of viral metagenomic reads obtained from Ion Torrent sequencing with the intent of improving downstream alignment/BLAST characterization. SPades (v3.8.0) output creates contigs.fasta and scaffolds.fasta files. Do these files contain all sequences found in the input file, even if they are not assembled, or only the sequences that are part of larger assembled contigs?
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I would say yes but I'm not sure.