no-discordant option in tophat · Biostar

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no-discordant option in tophat

Hi,

I want to use tophat for aligning paired-end reads from RNA-seq but I have had a lot of troubles with the program failing to report the output (seems like a common problem, several posts out there from people encountering the same issue), however I now managed to get around that problem by removing the --no-discordant flag, but my question is, how much does that decrease the quality of my output?

Frida

tophat alignment rna-seq

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updated 10.1 years ago by Biostar

• written 10.2 years ago by frida.danielsson •

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