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fasta to fastq

Hi guys,

I' m working with TopHat and Bowtie to start my Phaseolus' RNAseq analysis, but to use this protocol I need to convert the cDNA sequences .fasta to .fastq. I know the fasta sequence is more informative but the protocol use this kind of file.

What software can I use?

rna-seq tophat bowtie nature

1 answer

No, fastq is more informative, it includes base quality values.

But tophat will take fasta or fastq files, and so will bowtie, so you don't need to convert them.

See the manuals pages for Tophat and Bowtie.

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