This is a test version of Biostars. For the public version, visit https://www.biostars.org.
finding up or down regulated gene expression

I have read many questions related to this answer but still I doubt about it. my data looks like below 

              Treatment     Controlled 
Gene 1    0.23              0.48
Gene 2    0.65              0.76
Gene 3    0.88               0.66
Gene 4     0.48             0.065
....

I don't have any replicate and I want to find which gene is up regulated and which one is down regulated. I do know that I cannot get much info when i don't have any replicate but I want to know if there is any way to get up/down regulated genes based on statistical test ?

for example what if I get the fold change (treatment/controlled) and then I put a cutoff and select the genes based on that ?

r limma gene expression
• 2,269 views • link
updated by WouterDeCoster WouterDeCoster • written by Learner Learner

1 answer

Without replicates there is no valid statistical test which can reliably give you results about differential gene expression.

• 1 views • link
written by WouterDeCoster WouterDeCoster

@decosterwouter this is obvious ! please read above, these stuff are said million times. I am looking for another solution instead hearing what is already said over 10 times

• 1 views • link
written by Learner Learner

Log in to answer this question.

More posts like this