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Qiime Error in Split Libraries

Hello,

I am trying to use split libraries in qiime. The mapping files are in the folder I write, but I am getting this error and am not sure why: 

*split_libraries_fastq.py -i High\ merged/*_merged.fastq --sample_id 44881102 -o split_lib_44881102/ -m 44881102_mapping.txt --barcode_type 'not-barcoded' 

Error in split_libraries_fastq.py: No filepaths match pattern/name '44881102_mapping.txt'. All patterns must be matched at least once.*

Does anyone know what this is happening?

Thanks

qiime split libraries
• 3,697 views • link
written by espop23 espop23

2 answers

File path error, Can you copy the global file path for 44881102_mapping.txt?

If possible, replace -m 44881102_mapping.txt with -m ~/$PATHTODIR/44881102_mapping.txt

• 1 views • link
written by moore.jeremiah.mark • moore.jeremiah.mark

As in /Users//Documents/Data/High merged/44881102_mapping.txt ? I tried that and it comes up with the same error... Any suggestions?

• 1 views • link
written by espop23 espop23

Are you quoting or escaping spaces in your filepath?

• 1 views • link
written by fanli.gcb fanli.gcb

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