This is a test version of Biostars. For the public version, visit https://www.biostars.org.
GenBank conflicting annotations (identical proteins with different exons)

Here is the RefSeq mitochondrial complete genome record of Daucus carota

DEFINITION  Daucus carota subsp. sativus mitochondrion, complete genome.
ACCESSION   NC_017855
VERSION     NC_017855.1  GI:386799229
DBLINK      BioProject: PRJNA162951

It lists 2 identical proteins (NADH dehydrogenase subunit 5):

CDS         complement(join(220476..220625,221826..222220,
            51521..51542,33278..34495,35349..35576))
            /gene="nad5"
            /locus_tag="DacassM_p009"
            /trans_splicing
            /codon_start=1
            /product="NADH dehydrogenase subunit 5"
            /protein_id="YP_006291783.1"

CDS         complement(join(267858..268007,269208..269602,
            51521..51542,33278..34495,35349..35576))
            /gene="nad5"
            /locus_tag="DacassM_p008"
            /trans_splicing
            /codon_start=1
            /product="NADH dehydrogenase subunit 5"
            /protein_id="YP_006291784.1"

The proteins are 100% identical, though exons are different. It doesn't seem right to me. How is this possible?

genbank genome annotation refseq

1 answer

If you look into the gene anntotation you see that the first one is also annotated as 'copy 2'. Repeats are very common in plant mitochondrial genomes, maybe you should have a look into the respective paper at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413510/.

Log in to answer this question.