This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Visualize RSEM Results/Normalization

So I ran RSEM and got some data. I just want to check and make sure that my FPKM values are normalized. So I used excel (bar graph) and plotted the log2(FPKM+1). What I got ended up being half of a bell curve, with all values equal or greater to 0. This makes sense in my mind but I would just like to confirm that this process is right or if there is a better way of confirming that the results are normalized correctly. Thank you!

rna-seq rsem normalization
• 2,874 views • link
updated by Biostar Biostar • written by yrc0313 • yrc0313

1 answer

What does log2(FPKM+0.1) look like? Including smaller values should round out your bell curve.

• 0 views • link
written by Charles Warden Charles Warden

Log in to answer this question.

More posts like this