That one's really helpful, thank you very much！
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gerrychang1985 •
How to filter the raw data for RPKM calculation
Hi everyone,
I'm going to do some gene expression analysis recently. I plan to de novo assembly the transcripts, and then calculate the RPKM. But I'm confused by the filtration of raw data. When dealing with DNA reads, I know the PCR-duplication should be filtered out, but when coming to RNA expression, should I keep them?
Please help, thank you.
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Istvan Albert
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gerrychang1985 •
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gerrychang1985 •
1 answer
I would recommend reading the post:
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Istvan Albert
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