Hai,

Iam a Bioinfo student strating my Phd in Metagenomics.. My work is on the marine samples.. What would be the initial steps involved? My knowledge is limited in Bio technical aspect.. I see we must do 16s RNA isolation, then cloning to vector and then sequencing.. Can anyone suggest me some some specific methods to carry out these process i.e Which cloning vector is preferable ( Fosmid or BAC) and which which sequencing method can be used ( pyro or short gun) what are merits and limitations in these process? Your reply is awaited and appriciated.

regards Vishnu