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Different values of K

We have used SSR marker data to do population analysis with 330 and other with 325 samples. the 5 samples removed were duplicates, already represented in the 325 dataset. but with the structure harvester results the estimate of k is coming different in both case to be 5 and 4 respectively. Now we are unable to infer as to how by removing 5 duplicate samples the result can change?

PS: both runs were ran with similar parameters.

structure population genetics

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