Help with 2nd read data analysis
Analysing 2nd read from a V3 run on a HiSeq and I've goot the below image about quality:
Could anyone let me know if it is ok to trim the last 5-10 bases? The data woul still be usefull?
Many thanks
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Devon Ryan
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jmirobla •
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jmirobla •
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Sure, you can do a bit of quality trimming if it helps. The quality isn't really that bad (anything above 5 or so is useful for most things).
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No Image showing up. I'd advise you check this out, for common explanations around RNA Seq data
I fixed the image link.
Still no go on the image.
I wonder if every IP gets different hash. The current URL that works for me is http://s9.postimg.org/419yhqedp/2nd_read_quality.png
For reference: I am only seeing "enter image description here" in the original post. I can see the image at the link you posted.
Weird, they're identical links. I suspect that postimg is doing something on the back end to make it hard to directly link an image.
Either that or some quirk with Biostars code. I am going to do a test and post the same link here.
Image.
Edit: Does not get inserted into post using the "image" tag but a regular hyperlink works fine.
Weird, I see the image. When you click on the link, do you go directly to an image or to a webpage?
If I click on the link I go directly to the image.
Second line (image word) in my post has the same link with "image" tags and that does not work.
Update: It looked like biostars code had added a "!' before the Image tag. Taking that out made it work.