I have an assignment where we need to answer several questions about an EST, however, I'm having trouble getting started. My sequence contained a Not I site (clipped that) and no poly A signal.

We know that the sequence comes from the Honey Bee, so I used BLASTn to find a gene which I got a match for. The problem is I also performed a RNA refseq BLASTn using the EST and got 2 RNA variants of a totally different gene. The E values were 0 for both searches. How do I know which one is right?

Thanks for the help!