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Errors when downloading SRA data using fastq-dump

Hello,

I use fastq-dump in SRAToolkit to download raw data for RNA-seq analysis

My command line is: fastq-dump --split-files SRR1819888 & (I submit it to the background).

Then I always get error report from ncbi: ncbi_error_report.xml

Inside is :

</repository> <tools> <ascp> <max_rate>"1000m"</max_rate> </ascp> </tools> <vdb> <lib> <paths> <kfg>"/disk3/zhangjunhong/opt/SRA_Toolkit/bin"</kfg> </paths> </lib> </vdb> </config> <remoteaccess available="true"/> <currentprotectedrepository found="false"/> </configuration> <object path="SRR1557039" type="table" fs_type="unexpected"> </object> <software> <vdblibrary vers="2.7.8"/> <build static="f &lt;Er &lt;Error rc=" rc(rccont,rcnamelist,rcinserting,rcstring,rcempty)"="" function="VDBManagerListExternalSchemaModules"/> </build> <tool date="Dec 21 2015" name="fastq-dump" vers="2 &lt;Bin &lt;Binary path=" disk3="" zhangjunhong="" opt="" sra_toolkit="" bin="" fastq-dump"="" type="alias" md5="b8a9729b9fde6a7301467f7154db 40"> <alias resolved="fastq-dump.2"> <alias resolved="fastq-dump.2.5.7"/> </alias> </binary> </tool> </software> <env> </env> </report>

I have done homework and troubleshooting but still I have no ideal with this.

<object path="SRR1557039" type="table" fs_type="unexpected">

Do you have idea about this?

sequencing
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updated by Shicheng Guo Shicheng Guo • written by qingxiangg • qingxiangg

1 answer

I tried 1 minutes ago with your command: fastq-dump --split-files SRR1819888 &

And everything works fine. You can try to

1, Install lastest fastq-dump (fastq-dump : 2.5.5 works well)

2, check firewall

3, show your OS

Try again and provide more informations so that others can help you.

• 2 views • link
written by Shicheng Guo Shicheng Guo

Thanks Shicheng! Sorry for my late response! In fact I've solved this problem by switching to use aspera for downloading SRA data with command like this:

ascp –i ~/asperaweb_id_dsa.openssh –k 1 –T –l 200m anonftp@ftp.ncbi.nlm.nih.gov:/sra/sra-instant/reads/ByRun/sra/SRR/SRR304/SRR304976/SRR304976.sra ./

And then convert the SRA data to fastq using fastq-dump (SRA-toolkit):

fastq-dump –O output_directory --split-files SRR304976.sra

This works very well. And unfortunately, I still don't know what's wrong with my SRAToolkit.

And even SRAToolkit team admits that many bugs exist. Aspera works better with resuming function.

Anyway, I use SRAToolkit as converter now...

• 0 views • link
written by qingxiangg • qingxiangg

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