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SNP calling in non-model organism

hi I want to calling SNP in my RNAseq Data in my plant sample. my plant don't have reference genome. I de novo assembly my read. I want to use BWA and SAMTools softwares for SNP calling. Can anyone give me a good manual details for these two softwares?

snp

1 answer

See this SAMtools workflow, has all you asked for and more.

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