Hello,

I have a problem with blast db. I make it with makeblastdb with:

blastdbcmd -db nr -entry all -outfmt "%g %T" | \
  awk ' { if ($2 == 9606) { print $1 } } ' | \
  blastdbcmd -db refseq_protein -entry_batch - -out human_sequences.txt

from here

(dont know perl!)

and have this now:

psiblast -query query.txt -db db_prot -outfmt "7 qseqid sseqid evalue bitscore qlen gaps nident" -num_iterations 2 - max_target_seqs 20 -gilist human > seq0.txt>
**Error: NCBI C++ Exception:>
    "/build/buildd/ncbi-blast+-2.2.28/c++/src/corelib/ncbifile.cpp", line 4703: Error: ncbi::CMemoryFileMap::CMemoryFileMap() - To be memory mapped the file must exist:**

Can someone help ?

Regards