deal with the 16S rDNA sequence from ion torrent
Now I got some Fastq file from the ion torrent machine. And the person who give me this Fastq file tell me that the fastq doesn't have barcode , they are the v3 and v6 sequence from the fecal microorganism 16S pcr. I have to distinguish between V3 and V6, and use this file to try to use the PICRUSt(in QIIME).
I am totally a freshman in this. I have just tried the QIIME 454 tutorial, and Know the Mothur was used to deal with 16S sequence. Now, I am try to asking what should I do in this. Tell me whats the "begin"
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i find Mothur have a ion torrent butten link to an Example which show me how to analysis the ion torrent data( http://www.mothur.org/w/index.php?title=Ion_Torrent_sequence_analysis_using_Mothur&action=edit&redlink=1 ), but i click it, it show me with nothing... i want to know, where can i find the example~~~~(^-^!)
The result from the ion torrent machine are some fastq file. In the first line they have same title
@OJWK, so I use the bash commandssedandawk) to change their first line toand
and use bash command
catmake amerge.fastq, and use Qiime script(Convert) let the fastq file become two file== fna and qual. It means I split library by myself, but I dont filter the fastq, means they would have many low level sequence...my question: