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Pacbio is usefull for plants with big genome?

Hello everyone I wonder if anyone can help me

Can I use Pacbio to getting whole chloroplast sequencing for a plant with ~21Gb genome size? Just with a library and run on a smrtcell.

Thanks

sequencing

Hello Soorni, Aboozar!

We believe that this post does not fit the main topic of this site as this is not a bioinformatics question - please try SeqAnswers for sequencing related questions.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

I wouldn't necesarly have closed this. Despite the vague question the question of sequencing an organellar genome with pacbio has many bioinformatic and biological implications that are within this forum's boundries from what I have seen so far.

Anyways, I doubt that plant nuclear genome size has any relevance, since you are interested in the organellar genome, and those are usually never longer than 1 Mb, usually couple hundred kb. Although the smrtcell would certainly be useful to close gaps, you may need some additional sequencing with another technology perhaps to get a high quality consensus sequence. The question to answer is what is the % of reads that will be chloroplast and not nuclear, I suggest looking at other studies.

I told you why I closed it, it has nothing to do with bioinformatics and everything to do with sequencing strategy and library construction. Hence I closed it. As it happens you got an informative answer from someone at PacBio on SeqAnswers: http://seqanswers.com/forums/showthread.php?t=65357

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