Hi micro32uvas,

please have a look at the gff3 specifications (e.g. here). You can use command-line commands like awk to pick the columns of your file in the correct order. Alternatively, you can use Galaxy and click to "Text Manipulation->Cut columns from a table".

Denote, that some programs produces 0-based positions, which need to be converted for gff3 (1-based).

Cheers,

Michael