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Do the BLAST scores have any relation between them?
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2.6 years ago
Bahrani • 30
United States

Is there any relation among the BLAST scores (E-value, similarity, identity, gap, bit score)? Is the e-value score for an alignment proportional to other scores, such as similarity score (i.e. the lower the e-value, the higher the similarity)?

Thank you!

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15 months ago
Australia/Perth/UWA

The e-value is calculated by taking the score and including the number of sequences in your database, i.e., "what is the probability that I get this score with this many sequences?"

See previous discussions, https://www.biostars.org/p/9960/ or https://www.biostars.org/p/65025/

The score depends on the length of your alignment and your chosen substitution matrix, see these slides

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21 months ago
United States

Yes they do.

https://en.wikipedia.org/wiki/BLAST

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