How does edgeR normalize sequencing depth
Hi everybody!
I just started to use edgeR.And I am curious about the normalization methods used to normalize sequencing depth. But I can't find exact methods in edgeR UsersGuide. It only mentions TMM method that can adjust RNA composition.
Is there someone to help me?
Thank you very much!
1 answer
TMM is described here.
Yes, it deals with sequencing depth in a robust way.
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Sean Davis
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Sean Davis
TMM is the method used to normalize for sequencing depth. In fact, it works better than simply correcting by library size (see Figure 2 in the paper).
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