Hello

I know how to installed command line BLAST(2.2.31) previously, I installed and configured it on windows and unix environment (both are working perfectly). But this time I have large amount of sequences for blast therefor I thought to do this through remote server PuTTY / SSH .

I tried to download and configured it as I did in unix. BUT it seems that there is something wrong.

44227@lidong2:~>wget ftp://ftp.ncbi.nlm.nih.gov/blast/executables/LATEST/ncbi-blast-2.2.31+-X64-linux.tar.gz

I put it in a directory where I want to install and then extracted it and configured it as following:

$ export PATH="PATH:home/tb44227/nobackup/ncbi-blast-2.2.31+/bin

then I make the directory of db in ncbi-blast-2.2.31+ directory

$mkdir db

then downloaded the databases from ncbi and swissprot.

tb44227@lidong2:~/nobackup/ncbi-blast-2.2.31+> -bash: blastn: command not found
If '-bash:' is not a typo you can run the following command to lookup the package that contains the binary:
    command-not-found -bash:
-bash: -bash:: command not found

Could you please guide what|s wrong with the installation and configuration process of blast+.

I am absolutely new in SSH and PUTTY. Apologies if I made some mistakes.

Thanks