I have been looking at the bowtie2 and tophat2 manuals. Tophat2 (which calls bowtie2) can be run without specifying a phred scoring. In the documentation, it doesn't specify which is by default. So which is it?
I have used bowtie2 to map paired-end reads together with unpaired reads to a reference genome (default parameters). I noticed when looking at my alignment …
I am trying to tune my tophat2 runs following the example of [this][1] blog. They suggest trying different values for the `--mate-inner-dist` and `--mate-std-dev`. To …
i am a begginer user ,i install tophat2,bowtie2,fastqc and cufflink on desktop my 14.04 ubuntu by same these commands: tar -zxvf tophat-2.1.1.Linux_x86_64.tar.gz cd tophat-2.1.1.Linux_x86_64 ./tophat2 …
Dear All, I have a doubt, I am working on strand specific protocol (`fr-firststrand`). When aligning the reads using bowtie2, do I need to explicitly …
Is there any post/manual/blog to summarize the attribute tag (optional field) of tophat output bam file? How many attribute tags does tophat output bam file …
Hi everyone, I am trying to install `tophat-2.0.12.Linux_x86_64` and `bowtie2-2.2.3` on a server that has an older version of these software After unzipping the files …
I installed bowtie2 following the instructions given on http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml#getting-started-with-bowtie-2-lambda-phage-example When running the example though, I get the following error ``` Settings: Output files: "lambda_virus.*.bt2" Line …
<p>I am trying to use <a href='http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml#getting-started-with-bowtie-2-lambda-phage-example'>BowTie</a> for short read alignment on human genome. Now, after installing it I run the command of it's examples …