Thanks Ryan for your answer!
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kaczor.karolina •
RNA-Seq data validation by qPCR
Hi,
How could I validate the results of differentially expressed RNA by the use of RT-qPCR. What positive control should I use- is universal RNA good as a control for salivary RNA? Are other methods for validation of differentially expressed RNA? How to interpret the results of qPCR?
Thanks in advance
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kaczor.karolina •
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I've generally just ordered TaqMan primers, that's about the simplest method. I've never bothered with positive controls, they're not particularly useful. As for what to compare against, I can't give you any advice there, since I've never used salivary RNA.
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Devon Ryan
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