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How to figure out coding sequence based on the results of blastx

Hey guys,

I have annotated my sequences with Blast2go. Now I need to label the coding sequence (find out start and stop codon). How do I do it? Does Blast2go have this function?

By the way, after annotation, I've got the full gene names and gi numbers for each sequence. How do I find gene symbols for each sequence?

annotation coding-sequence
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updated by Ram Ram • written by ngs101.com ngs101.com

2 answers

Try running Augustus or ORF finder

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written by Prakki Rama Prakki Rama

If you did your analysis in de novo trascriptome assembly manner, I recommend "TransDecoder Tool" (https://transdecoder.github.io/), it is good in terms of quality and fastness.

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updated by Ram Ram • written by vahapel vahapel

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