using DEseq to produce RPKM and VST
Good morning friends,
At last I produced a file containing the reads raw counts. Now I should prepare VST and RPKM by DEseq, but I am not familiar with R. Only rstudio and DEseq package installed. Do you know any script or a way to start?
Thank you
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Is not this what you are looking for? A: Calculating RPKM for DESeq
If yes, than here is good work-through solution https://github.com/mgonzalezporta/TeachingMaterial/blob/master/doc/25.normalising.md
Read DESeq vignette. It's well written and easily understandable