Entering edit mode
8.5 years ago
Thomas Moody
▴
30
I'm trying to use the uparse_ref (http://www.drive5.com/usearch/manual/cmd_uparse_ref.html) command to annotate a set of reads from a MiSeq run. The database was generated by someone in my lab, and has worked in the past.
The issue I'm running into is that whenever I run the command, it hangs on the classifying step almost immediately after running, i.e. my terminal output freezes at
00:00 4.8Mb 0.1% Classifying
and I can't seem to do anything to overcome this. I've actually run a lab mate's pipeline (for whom the command worked) on my machine using all of their data, and the program still froze! Any ideas on how to move past this?
