Hello,

I'm using blast to retrieve sequence matches and I want the subject start and end coordinates. But, I notice that although the subject start returns a column, there is no column for the subject end field.

Here is the code I used:

blastn \
  -query Gaviaimmerheader.fasta \
  -db Gallus_gallus.Galgal4.cds.all.fa \
  -task blastn \
  -dust no \
  -outfmt "6 qseqid sseqid length qstart qend sstart send sallgi qseq sseq evalue" \
  -evalue 1e-6 \
  -num_alignments 1 \
  -max_hsps 5 \
  -out outputgallus8.blast.txt

If anyone knows why the send field is entirely absent, let me know.

Thanks,
Zach