What are the good tools for mRNA codon usage optimization and secondary structure prediction?
Hi all,
I'm looking for tool to do codon usage optimization for mRNAs and secondary structure prediction;
Basically I need to optimize codon usage to increase expression of the gene in host cell. Also I need to confirm that in the new sequence ribosome binding site are exposed and it's not involved in any secondary structure.
Would someone recommend some tools?
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You can use RNAup for your second task, it gives an accessibility value for each window.
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