Hello,
I used to work with microarray data. When I was beginning in the field I found several good and introductory reviews that helped a lot for someone with computer science background. For instance:
- Alvis Brazma, Jaak Vilo, Gene expression data analysis, FEBS Letters, Volume 480, Issue 1, 25 August 2000, Pages 17-24
- Christina A Harrington, Carsten Rosenow, Jacques Retief, Monitoring gene expression using DNA microarrays, Current Opinion in Microbiology
- Adi L. Tarca, Roberto Romero, Sorin Draghici, Analysis of microarray experiments of gene expression profiling, American Journal of Obstetrics and Gynecology
Can anyone recommend any similar introductory material regarding RNA-Seq?
All the best!
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NetunoPoncã
2 answers
You will get may if you search though google. There are plenty of tutorials and review papers available but you can start with NGS and then RNA-seq. Read few articles on RNA-Seq data analysis, like cuffdiff protocol (though many new tools have come, its still a good start). Once you understand the basic data analysis pipeline, start exploring the tools like HTSeq-count, edgeR/DESeq etc.
There are many threads in biostars as well. E.g: How Do I Get Started Working With Rna-Seq Data
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GouthamAtla
The following pages have information for both RNA-Seq and microarray analysis, but it may still be worth a quick look since I have tried to list the most popular programs used for various types of analysis:
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Charles Warden
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