Hey,

I am an amateur person interested in exploring my genome, I have finally got my Exome data, but I have found some strange result, for example there are some bases which despite having high depth (number of read count), still have something like 60/40 percent between two different reads, for example in 40 percent of reads it is T and in 60 percent it is C. Here is a sample out of my exome:

Location = chr15:73,852,467
Total count: 99
A      : 0
C      : 60  (61%,     31+,   29- )
G      : 0
T      : 39  (39%,     18+,   21- )
N      : 0

Can anyone tell me what it means to have such a result? is it really possible for sequencer to do 39 wrong readings (and all of them a specific result) in 99 tries?