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Plotting sequence similarity/identity of a multiple sequence alignment

Hello,

I have multiple sequence alignment files, some of NT some of AA. I wanted to plot similarity of these alignments along the coordinates of the alignment.

Basically the alignment should highlight regions that are very conserved among sequences and regions that are very divergent.

Something similar has been achieved here:

http://www.biology.wustl.edu/gcg/figure/plotsimilarity_1.gif

I am having no luck finding this in google.

Thanks, Adrian

msa r plotting

1 answer

PlotSimilarity from GCG .. brings back memories.

You could use plotcon from EMBOSS as a replacement: http://emboss.sourceforge.net/apps/release/6.6/emboss/apps/plotcon.html

Dot plots may also be an alternative.

Brilliant! This works perfectly.

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