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sequence files were removed by mothur?

When I used mothur to align my 16srRNA,

mothur > screen.seqs(fasta=stability1.trim.contigs.good.unique.align, count=stability1.trim.contigs.good.count_table, summary=stability1.trim.contigs.good.unique.summary, start=1968, end=11550, maxhomop=8)

It comes back:

Removing group: C_19-4 because all sequences have been removed.

Removing group: C_19pl6 because all sequences have been removed.

Anyone can suggest what is going on?

Thank you.

next-gen sequencing 16srrna

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