This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Tutorial: Determine if a transcription factor is bound with CENTIPEDE

I wrote a practical tutorial for how to use CENTIPEDE to determine if a transcription factor is bound to a site in the genome.

The tutorial provides step-by-step instructions for:

  1. Preparing the appropriate input data.
  2. Running the analysis.
chip-seq transcription-factor dnase-seq centipede

2 answers

Hi Kamil,

Thank you for producing this great tutorial.

I have a question. When I try to use the centipede_data function of the R package that you have provided, I always end up getting this error:

Error in sprintf("%.bai", bam_file) :
  invalid format '%.ba'; use format %s for character objects

Do you have any suggestion to solve this issue?

Thanks a lot.

Thanks for catching the error, now fixed.

Works perfectly.

Thank you once again for the effort to produce this great package. You have made it very easy to use CENTIPEDE.

Best Regards,

Chadi

Hi Kamil,

Thank you again for producing this fantastic tutorial.

When I use devtools::install_github("slowkow/CENTIPEDE.tutorial") to install the tutorial, I got this error:

Downloading GitHub repo slowkow/CENTIPEDE.tutorial@master
Error in curl::curl_fetch_memory(url, handle = handle) :
  couldn't resolve host name

Could you please help me with this problem? I searched it in many websites but still no answer.

Thank you. And early to wish you Merry Christmas and Happy New Year~

Are you working behind a university computer? If you are you'll probably need to set up a proxy to avoid the error.

library(httr)
set_config(use_proxy(url="proxy.xxxx.com", port=xxxx, username="user",password="password"))

Only fill in the username and password field if you need a username and password to use the proxy. Make sure you also have devtools and Rtools installed (can be installed via the install.packages("") function).

Thank you a lot.

I got this error:

package 'git2r' is not available (for R version 3.2.2)

Should I use the 2.* version?

Log in to answer this question.