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can anyone explain pipes in blastn output

Hi All,

Can anyone explain what does all the pipes and letters mean in the blastn (local blast, ncbi-blast+ on linux terminal)?

are those alternatives to the main solution?

Query_1  1381   GGCAATGCGGTGAACGCCATGTCTACCTATCTTCCCGTGCAAGACGGGACGTACCATCAG  1440
178688   9271   GGCAATGCGGTGAACGCCATGTCTACCTATCTTCCCGTGCAAGACGGGACGTACCATCAG  9330
97017    10239  --CAATGCGGTGAACGCCATGTCCGCATATCTTCCCGTGCAAGACGGGACGTACCATCAA  10173
                                            \ \\\   \                       
                                            | |||   |                       
                                           TT GAAT  C
                                               ||                           
                                               C|
                                                |                           
                                                C

Thanks,
Raz

blast

Formatting is making it hard to see what is being represented in the "code" part. What output format option was used to get this?

-outfmt 1

What is the problem with formatting? I can see clearly on my computer.

I think it just depends on the size of your monitor. Adjusting the zoom should allow you to visualise the alignment properly.

1 answer

Not sure if this link will come through but here goes: https://books.google.com/books?id=zDqksXUaoBAC&pg=PT312&lpg=PT312&dq=query-anchored+showing+identities

The representation may have changed with blast+ since the book refers to the older version of blast. Checking to see what the new outfmt '1' looks like now.

Great thanks!!

these are insertions that do not occur in the query seq. it became clear when I used outfmt 3

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