Ok, but I must use this tool called Pfam Scan:
http://www.ebi.ac.uk/Tools/pfa/pfamscan/
Thx it is the same output :P
Hi everyone!
I need your help with Pfam Scan output file. There is a lot of unclear columns and the official documentation have only information about output file formats...
Where I can find good documentation for this tool? Or someone can explain me the means of columns:
alignment start/end (I think that is place in my input sequence where alignment starts/ends)
envelope start/end (I really don't have idea)
hmm start/end (I don't know)
hmm length (sometimes is larger than hmm_end - hmm_start.......why?)
bit score (i think that if is big is better than small :P, but I can't interpret this value correctly (I don't know the scale))
significance (should I use only lines with significance==1? Why?)
Mainly: I need means of start/end points (and differences between them)
http://hmmer.janelia.org/help/result
Pfam is a database, the tool you can use to scan pfam is HMMER, as it says in the pfam publication: http://nar.oxfordjournals.org/content/42/D1/D222.long.
Ok, but I must use this tool called Pfam Scan:
http://www.ebi.ac.uk/Tools/pfa/pfamscan/
Thx it is the same output :P
Yep, as I said, if you read the publication I linked it will tell you that they use HMMER.
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